Abstract
Five new water-soluble derivatives of Nile Red 1-5 were prepared. These benzophenoxazine dyes fluoresce between 640 and 667 nm with quantum yields of 0.17-0.33 in pH 7.4 phosphate buffer, and at slightly shorter wavelengths and higher quantum yields in EtOH. Two dyes, 3 and 4 permeated into Clone 9 cells and selectively stained mitochondria and golgi, respectively.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cells, Cultured
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / metabolism*
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Molecular Structure
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Organelles / chemistry*
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Organelles / metabolism*
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Oxazines / chemical synthesis
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Oxazines / chemistry*
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Oxazines / metabolism*
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Quantum Theory
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Solubility
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Staining and Labeling
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Tissue Distribution
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Water / chemistry*
Substances
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Fluorescent Dyes
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Oxazines
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Water