Ventral tegmental area leptin receptor neurons specifically project to and regulate cocaine- and amphetamine-regulated transcript neurons of the extended central amygdala

J Neurosci. 2010 Apr 21;30(16):5713-23. doi: 10.1523/JNEUROSCI.1001-10.2010.

Abstract

Leptin acts via its receptor (LepRb) to regulate neural circuits in concert with body energy stores. In addition to acting on a number of hypothalamic structures, leptin modulates the mesolimbic dopamine (DA) system. To determine the sites at which LepRb neurons might directly influence the mesolimbic DA system, we examined the distribution of LepRb neurons and their projections within mesolimbic brain regions. Although the ventral tegmental area (VTA) contains DA LepRb neurons, LepRb neurons are absent from the amygdala and striatum. Also, LepRb-EGFPf mice (which label projections from LepRb neurons throughout the brain) reveal that few LepRb neurons project to the nucleus accumbens (NAc). In contrast, the central amygdala (CeA) and its rostral extension receive copious projections from LepRb neurons. Indeed, LepRb-specific anterograde tracing demonstrates (and retrograde tracing confirms) that VTA LepRb neurons project to the extended CeA (extCeA) but not the NAc. Consistently, leptin promotes cAMP response element-binding protein phosphorylation in the extCeA, but not NAc, of leptin-deficient animals. Furthermore, transgenic mice expressing the trans-synaptic tracer wheat germ agglutinin in LepRb neurons reveal the innervation of CeA cocaine- and amphetamine-regulated transcript (CART) neurons by LepRb neurons, and leptin suppresses the increased CeA CART expression of leptin-deficient animals. Thus, LepRb VTA neurons represent a subclass of VTA DA neurons that specifically innervates and controls the extCeA; we hypothesize that these neurons primarily modulate CeA-directed behaviors.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amphetamine* / analysis
  • Amygdala / chemistry
  • Amygdala / physiology*
  • Animals
  • Cocaine* / analysis
  • Mice
  • Mice, Obese
  • Mice, Transgenic
  • Neural Pathways / chemistry
  • Neural Pathways / physiology
  • Neurons / chemistry
  • Neurons / classification
  • Neurons / physiology*
  • Receptors, Leptin / analysis
  • Receptors, Leptin / physiology*
  • Transcription, Genetic / physiology
  • Ventral Tegmental Area / chemistry
  • Ventral Tegmental Area / physiology*

Substances

  • Receptors, Leptin
  • Amphetamine
  • Cocaine