Valosin-containing protein (VCP) in novel feedback machinery between abnormal protein accumulation and transcriptional suppression

J Biol Chem. 2010 Jul 9;285(28):21736-49. doi: 10.1074/jbc.M109.099283. Epub 2010 Apr 21.

Abstract

Abnormal protein accumulation is often observed in human neurodegenerative disorders such as polyglutamine diseases and Parkinson disease. Genetic and biochemical analyses indicate that valosin-containing protein (VCP) is a crucial molecule in the pathogenesis of human neurodegenerative disorders. We report here that VCP was specifically modified in neuronal cells with abnormal protein accumulation; this modification caused the translocation of VCP into the nucleus. Modification-mimic forms of VCP induced transcriptional suppression with deacetylation of core histones, leading to cell atrophy and the decrease of de novo protein synthesis. Preventing VCP nuclear translocation in polyglutamine-expressing neuronal cells and Drosophila eyes mitigated neurite retraction and eye degenerations, respectively, concomitant with the recovery of core histone acetylation. This represents a novel feedback mechanism that regulates abnormal protein levels in the cytoplasm during physiological processes, as well as in pathological conditions such as abnormal protein accumulation in neurodegenerations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • Adenosine Triphosphatases / chemistry
  • Adenosine Triphosphatases / physiology*
  • Animals
  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / physiology*
  • Cell Line
  • Drosophila melanogaster / genetics
  • Genetic Vectors
  • Histones / chemistry
  • Humans
  • Mice
  • Mice, Transgenic
  • NIH 3T3 Cells
  • Neurodegenerative Diseases / metabolism
  • Neurons / metabolism
  • PC12 Cells
  • Peptides / genetics
  • Peptides / metabolism
  • Rats
  • Transcription, Genetic*
  • Valosin Containing Protein

Substances

  • Cell Cycle Proteins
  • Histones
  • Peptides
  • polyglutamine
  • Adenosine Triphosphatases
  • VCP protein, human
  • Valosin Containing Protein
  • Vcp protein, mouse
  • Vcp protein, rat