The performance of the recently developed Seeplex VRE detection kit was compared with chromID VRE agar and the conventional blood agar plate with vancomycin disk (BAP-VA) for detection of vancomycin-resistant enterococci (VRE) from 180 rectal swab specimens. After 18 hr enrichment in enterococcosel broth (EB), the sensitivity and specificity for detection of vancomycin-resistant E. faecium were as follows: Seeplex VRE detection kit, 92.5% and 98.9%; chromID VRE agar, 92.5% and 97.8%; BAP-VA, 77.5% and 70.3%, respectively. The reporting times averaged 2, 3.4, and 3.6 days, respectively. The Seeplex VRE detection kit and chromID VRE agar were comparable and were superior to BAP-VA. Unexpectedly, 15.7% of VRE were vanA-positive Enterococcus species other than E. faecium and E. faecalis. Vigilance is suggested to see if these species eventually emerge as significant pathogens.