A dibasic amino acid pair conserved in the activation loop directs plasma membrane localization and is necessary for activity of plant type I/II phosphatidylinositol phosphate kinase

Plant Physiol. 2010 Jul;153(3):1004-15. doi: 10.1104/pp.109.152686. Epub 2010 Apr 28.

Abstract

Phosphatidylinositol phosphate kinase (PIPK) is an enzyme involved in the regulation of cellular levels of phosphoinositides involved in various physiological processes, such as cytoskeletal organization, ion channel activation, and vesicle trafficking. In animals, research has focused on the modes of activation and function of PIPKs, providing an understanding of the importance of plasma membrane localization. However, it still remains unclear how this issue is regulated in plant PIPKs. Here, we demonstrate that the carboxyl-terminal catalytic domain, which contains the activation loop, is sufficient for plasma membrane localization of PpPIPK1, a type I/II B PIPK from the moss Physcomitrella patens. The importance of the carboxyl-terminal catalytic domain for plasma membrane localization was confirmed with Arabidopsis (Arabidopsis thaliana) AtPIP5K1. Our findings, in which substitution of a conserved dibasic amino acid pair in the activation loop of PpPIPK1 completely prevented plasma membrane targeting and abolished enzymatic activity, demonstrate its critical role in these processes. Placing our results in the context of studies of eukaryotic PIPKs led us to conclude that the function of the dibasic amino acid pair in the activation loop in type I/II PIPKs is plant specific.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids, Diamino / chemistry*
  • Animals
  • Arabidopsis / cytology
  • Arabidopsis / drug effects
  • Arabidopsis / enzymology
  • Bryopsida / drug effects
  • Bryopsida / enzymology*
  • Catalytic Domain
  • Cell Membrane / drug effects
  • Cell Membrane / enzymology*
  • Conserved Sequence*
  • Enzyme Activation / drug effects
  • Molecular Sequence Data
  • Onions / cytology
  • Onions / drug effects
  • Onions / enzymology
  • Phosphatidic Acids / pharmacology
  • Phosphotransferases (Alcohol Group Acceptor) / chemistry*
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism*
  • Protein Transport / drug effects
  • Protoplasts / drug effects
  • Protoplasts / enzymology
  • Structure-Activity Relationship
  • Subcellular Fractions / drug effects
  • Subcellular Fractions / enzymology

Substances

  • Amino Acids, Diamino
  • Phosphatidic Acids
  • Phosphotransferases (Alcohol Group Acceptor)
  • 1-phosphatidylinositol-4-phosphate 5-kinase