Comparative gene expression analysis in mouse models for multiple sclerosis, Alzheimer's disease and stroke for identifying commonly regulated and disease-specific gene changes

Genomics. 2010 Aug;96(2):82-91. doi: 10.1016/j.ygeno.2010.04.004. Epub 2010 May 7.

Abstract

The brain responds to injury and infection by activating innate defense and tissue repair mechanisms. Working upon the hypothesis that the brain defense response involves common genes and pathways across diverse pathologies, we analysed global gene expression in brain from mouse models representing three major central nervous system disorders, cerebral stroke, multiple sclerosis and Alzheimer's disease compared to normal brain using DNA microarray expression profiling. A comparison of dysregulated genes across disease models revealed common genes and pathways including key components of estrogen and TGF-beta signaling pathways that have been associated with neuroprotection as well as a neurodegeneration mediator, TRPM7. Further, for each disease model, we discovered collections of differentially expressed genes that provide novel insight into the individual pathology and its associated mechanisms. Our data provide a resource for exploring the complex molecular mechanisms that underlie brain neurodegeneration and a new approach for identifying generic and disease-specific targets for therapy.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / genetics
  • Alzheimer Disease / metabolism*
  • Animals
  • Brain / metabolism*
  • Gene Expression Regulation / genetics
  • Gene Expression Regulation / physiology*
  • Mice
  • Mice, Transgenic
  • Multiple Sclerosis / genetics
  • Multiple Sclerosis / metabolism*
  • Oligonucleotide Array Sequence Analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction / genetics*
  • Stroke / genetics
  • Stroke / metabolism*
  • Systems Biology / methods
  • TRPM Cation Channels / metabolism

Substances

  • TRPM Cation Channels
  • Trpm7 protein, mouse