Protection against Helicobacter pylori infection by a trivalent fusion vaccine based on a fragment of urease B-UreB414

J Microbiol. 2010 Apr;48(2):223-8. doi: 10.1007/s12275-009-0233-4. Epub 2010 May 1.

Abstract

A multivalent fusion vaccine is a promising option for protection against Helicobacter pylori infection. In this study, UreB414 was identified as an antigenic fragment of urease B subunit (UreB) and it induced an antibody inhibiting urease activity. Immunization with UreB414 partially protected mice from H. pylori infection. Furthermore, a trivalent fusion vaccine was constructed by genetically linking heat shock protein A (HspA), H. pylori adhesin A (HpaA), and UreB414, resulting in recombinant HspA-HpaA-UreB414 (rHHU). Its protective effect against H. pylori infection was tested in BALB/c mice. Oral administration of rHHU significantly protected mice from H. pylori infection, which was associated with H. pylori-specific antibody production and Th1/Th2-type immune responses. The results show that a trivalent fusion vaccine efficiently combats H. pylori infection, and that an antigenic fragment of the protein can be used instead of the whole protein to construct a multivalent vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Bacterial / genetics
  • Adhesins, Bacterial / immunology*
  • Administration, Oral
  • Animals
  • Antibodies, Bacterial / blood
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology*
  • Bacterial Vaccines / administration & dosage
  • Bacterial Vaccines / genetics
  • Bacterial Vaccines / immunology*
  • Female
  • Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / immunology*
  • Helicobacter Infections / immunology
  • Helicobacter Infections / prevention & control*
  • Helicobacter pylori / genetics
  • Helicobacter pylori / immunology*
  • Interferon-gamma / biosynthesis
  • Interleukin-4 / biosynthesis
  • Lymphocytes / immunology
  • Mice
  • Mice, Inbred BALB C
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Stomach / microbiology
  • Stomach / pathology
  • Urease / genetics
  • Urease / immunology*
  • Virulence Factors / genetics
  • Virulence Factors / immunology

Substances

  • Adhesins, Bacterial
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Proteins
  • Bacterial Vaccines
  • Heat-Shock Proteins
  • HspA protein, bacteria
  • Recombinant Fusion Proteins
  • Virulence Factors
  • adhesin A, Helicobacter
  • Interleukin-4
  • Interferon-gamma
  • Urease