Plasmodium vivax: comparison of the immune responses between oral and parenteral immunization of rPv54 in BALB/c mice

Myoung-Hee Kwon; Hyung-Hwan Kim; Ho-Sa Lee; Tong-Soo Kim; Chang-Mi Oh; Yong-Joo Ahn; Seo-Kyong Hwang; Youngjoo Sohn; Hyuck Kim; Hyeong-Woo Lee

doi:10.1016/j.exppara.2010.05.001

Plasmodium vivax: comparison of the immune responses between oral and parenteral immunization of rPv54 in BALB/c mice

Exp Parasitol. 2010 Oct;126(2):217-23. doi: 10.1016/j.exppara.2010.05.001. Epub 2010 May 9.

Authors

Myoung-Hee Kwon¹, Hyung-Hwan Kim, Ho-Sa Lee, Tong-Soo Kim, Chang-Mi Oh, Yong-Joo Ahn, Seo-Kyong Hwang, Youngjoo Sohn, Hyuck Kim, Hyeong-Woo Lee

Affiliation

¹ Division of Malaria and Parasitic Diseases, National Institute of Health, Centers for Disease Control and Prevention, Seoul 122-701, Republic of Korea.

PMID: 20460123
DOI: 10.1016/j.exppara.2010.05.001

Abstract

The merozoite surface protein-1 (MSP-1) from Plasmodium vivax was evaluated as an oral vaccine candidate by cloning and expressing the interspecies conserved block 10 (ICB10) of the MSP-1 from a Korean isolate in Escherichia coli. The expressed fusion protein contained ICB10 and a maltose-binding protein (MBP), rPv54, has a molecular weight of approximately 54 kDa as determined by SDS-PAGE analysis. IgG against rPv54 was successfully produced in BALB/c mice by oral immunization and sustained for more than 4 months. IgG2b was dominantly produced in both oral and parenteral immunizations. The rPv54 increased the frequency of NK, NKT, CD4+ T, CD8+ T, and B cells in both immunizations. IL-5 and TNF-alpha were increased in both significantly. In conclusion, rPv54 might be a valuable potential vaccine candidate for the oral and parenteral immunization against vivax malaria.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Administration, Oral
Animals
Antibodies, Protozoan / biosynthesis
Female
Humans
Immunoglobulin G / biosynthesis
Infusions, Parenteral
Interleukin-5 / metabolism
Malaria, Vivax / prevention & control
Maltose-Binding Proteins
Merozoite Surface Protein 1 / chemistry
Merozoite Surface Protein 1 / genetics
Merozoite Surface Protein 1 / immunology*
Mice
Mice, Inbred BALB C
Periplasmic Binding Proteins / chemistry
Periplasmic Binding Proteins / genetics
Periplasmic Binding Proteins / immunology*
Plasmodium vivax / immunology*
Protozoan Vaccines / administration & dosage*
Protozoan Vaccines / immunology
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / immunology*
Spleen / cytology
Spleen / immunology
Tumor Necrosis Factor-alpha / metabolism
Vaccines, Synthetic / administration & dosage
Vaccines, Synthetic / immunology

Substances

Antibodies, Protozoan
Immunoglobulin G
Interleukin-5
Maltose-Binding Proteins
Merozoite Surface Protein 1
Periplasmic Binding Proteins
Protozoan Vaccines
Recombinant Fusion Proteins
Tumor Necrosis Factor-alpha
Vaccines, Synthetic