Importance of TLR2 in the direct response of T lymphocytes to Schistosoma mansoni antigens

Eur J Immunol. 2010 Aug;40(8):2221-9. doi: 10.1002/eji.200939998.

Abstract

The immunomodulatory effect of Schistosoma mansoni antigens has often been attributed to interaction with PRR expressed on APC. Our previous work has shown that S. mansoni-soluble egg antigen (SEA) can induce, together with a Th2 response, TGF-β-dependent Foxp3 expression in naïve CD4(+) T cells from NOD mice. We found that SEA can directly upregulate the expression of surface-bound TGF-β in purified CD4(+) T cells in the absence of accessory cell interactions. In this study, we show that the C-type lectin receptors DEC-205 and galectin-3 were involved in the direct interaction between S. mansoni antigens and CD4(+) T cells. SEA was able to enhance CD4(+) T-cell secretion of bioactive TGF-β in response to TLR2 ligand stimulation, in the absence of APC. We also show that TLR2 expressed on CD4(+) T cells was important for the Foxp3 expression induced by SEA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / immunology
  • Antigens, CD / metabolism
  • Antigens, Helminth / immunology*
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / metabolism*
  • CD4-Positive T-Lymphocytes / parasitology
  • CD4-Positive T-Lymphocytes / pathology
  • Cells, Cultured
  • Female
  • Forkhead Transcription Factors / biosynthesis*
  • Forkhead Transcription Factors / genetics
  • Galectin 3 / immunology
  • Galectin 3 / metabolism
  • Helminth Proteins / immunology*
  • Host-Pathogen Interactions
  • Immunomodulation
  • Lectins, C-Type / immunology
  • Lectins, C-Type / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred NOD
  • Mice, Knockout
  • Minor Histocompatibility Antigens
  • Receptors, Cell Surface / immunology
  • Receptors, Cell Surface / metabolism
  • Schistosoma mansoni / immunology*
  • Toll-Like Receptor 2 / genetics
  • Toll-Like Receptor 2 / immunology
  • Toll-Like Receptor 2 / metabolism*
  • Transforming Growth Factor beta / biosynthesis
  • Transforming Growth Factor beta / genetics
  • Transforming Growth Factor beta / metabolism

Substances

  • Antigens, CD
  • Antigens, Helminth
  • DEC-205 receptor
  • Forkhead Transcription Factors
  • Foxp3 protein, mouse
  • Galectin 3
  • Helminth Proteins
  • Lectins, C-Type
  • Minor Histocompatibility Antigens
  • Receptors, Cell Surface
  • Tlr2 protein, mouse
  • Toll-Like Receptor 2
  • Transforming Growth Factor beta
  • gp50 protein, Schistosoma