Exploration of disease mechanism in acute kidney injury using a multiplex bead array assay: a nested case-control pilot study

Biomarkers. 2010 Aug;15(5):436-45. doi: 10.3109/1354750X.2010.485252.

Abstract

Background: Acute kidney injury (AKI) following cardiac surgery with cardiopulmonary bypass (CPB) causes increased morbidity and mortality.

Objective: To evaluate the plasma profile of biomarkers potentially involved in AKI development following CPB.

Methods: In a nested case-control study, plasma levels of 27 biomarkers in 11 AKI cases were compared with 25 controls.

Results: Pre-CPB, plasma levels of epidermal growth factor and macrophage inflammatory protein-1beta, 2 h following CPB, soluble vascular cell adhesion molecule-1 (sVCAM-1), fractalkine and macrophage inflammatory protein-1alpha, and at later time points, sVCAM-1 and interleukin-6 were associated with AKI.

Conclusion: Biomarkers associated with AKI following CPB may merit further study.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Kidney Injury / blood*
  • Acute Kidney Injury / diagnosis
  • Acute Kidney Injury / surgery
  • Aged
  • Aged, 80 and over
  • Biomarkers / blood*
  • Cardiopulmonary Bypass
  • Case-Control Studies
  • Chemokine CCL3 / blood
  • Chemokine CCL4 / blood
  • Chemokine CX3CL1 / blood
  • Epidermal Growth Factor / blood
  • Female
  • Humans
  • Immunoassay / instrumentation
  • Immunoassay / methods*
  • Interleukin-6 / blood
  • Male
  • Middle Aged
  • Monitoring, Physiologic / methods*
  • Pilot Projects
  • Prospective Studies
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Time Factors
  • Treatment Outcome
  • Vascular Cell Adhesion Molecule-1 / blood

Substances

  • Biomarkers
  • Chemokine CCL3
  • Chemokine CCL4
  • Chemokine CX3CL1
  • Interleukin-6
  • Vascular Cell Adhesion Molecule-1
  • Epidermal Growth Factor