Gankyrin promotes the proliferation of human pancreatic cancer

Yun Meng; Lijie He; Xuegang Guo; Shanhong Tang; Xiaodi Zhao; Rui Du; Jiang Jin; Qian Bi; Hao Li; Yongzhan Nie; Jie Liu; Daiming Fan

doi:10.1016/j.canlet.2010.04.019

Gankyrin promotes the proliferation of human pancreatic cancer

Cancer Lett. 2010 Nov 1;297(1):9-17. doi: 10.1016/j.canlet.2010.04.019. Epub 2010 May 18.

Authors

Yun Meng¹, Lijie He, Xuegang Guo, Shanhong Tang, Xiaodi Zhao, Rui Du, Jiang Jin, Qian Bi, Hao Li, Yongzhan Nie, Jie Liu, Daiming Fan

Affiliation

¹ Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, China.

PMID: 20483533
DOI: 10.1016/j.canlet.2010.04.019

Abstract

Previous studies in our laboratory have suggested that gankyrin expression is correlated with a malignant phenotype in colorectal cancer. Here, we investigated the possible role of gankyrin in pancreatic carcinogenesis. Gankyrin expression was significantly increased in pancreatic cancer compared to non-cancerous tissues. This expression significantly enhanced cancer cell proliferation and growth in vitro and in vivo. Suppression of gankyrin downregulated cyclin A, cyclin D1, cyclin E, CDK2, CDK4, PCNA and p-Rb but upregulated p27, Rb and p53. However, gankyrin overexpression led to opposite results. Thus, gankyrin could enhance pancreatic cancer cell proliferation by promoting cell cycle progression and p53 degradation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Cycle*
Cell Line, Tumor
Cell Proliferation*
Cyclin A / metabolism
Cyclin D1 / metabolism
Cyclin E / metabolism
Cyclin-Dependent Kinase 2 / metabolism
Cyclin-Dependent Kinase 4 / metabolism
Cyclin-Dependent Kinase Inhibitor p27 / metabolism
Female
Humans
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Middle Aged
Neoplasm Staging
Pancreatic Neoplasms / genetics
Pancreatic Neoplasms / metabolism*
Pancreatic Neoplasms / pathology
Phosphorylation
Proliferating Cell Nuclear Antigen / metabolism
Proteasome Endopeptidase Complex / genetics
Proteasome Endopeptidase Complex / metabolism*
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins / metabolism*
RNA Interference
Retinoblastoma Protein / metabolism
Time Factors
Transfection
Tumor Burden
Tumor Suppressor Protein p53 / metabolism
Up-Regulation

Substances

CCND1 protein, human
Cyclin A
Cyclin E
PSMD10 protein, human
Proliferating Cell Nuclear Antigen
Proto-Oncogene Proteins
Retinoblastoma Protein
TP53 protein, human
Tumor Suppressor Protein p53
Cyclin D1
Cyclin-Dependent Kinase Inhibitor p27
CDK2 protein, human
CDK4 protein, human
Cyclin-Dependent Kinase 2
Cyclin-Dependent Kinase 4
Proteasome Endopeptidase Complex