Some yeasts are preferred to bacteria as sources for biosurfactants, mainly due to their GRAS status for environmental and health safety reasons. This chapter thus focuses on the production of biosurfactants by some yeast cultures using renewable resources like fatty wastes from household and vegetable oil refineries as major substrates. The chapter also emphasizes on the importance of the application of response surface methodology and artificial neural network techniques for the optimization of biosurfactant production by yeasts.