Abstract
Dicistroviruses have motifs for picornavirus 2C, 3C, and 3D proteins in their nonstructural polyprotein C-terminal region. The proteins from the nonstructural, N-terminal region of the polyprotein remain to be characterized. We have identified 3C-mediated cleavage sites in the N-terminal region of the nonstructural polyprotein of the dicistrovirus Plautia stali intestine virus (PSIV). The 2B/2C cleavage site mapped to amino acids (aa) 408-409 (QD). 2B/2C cleavage sites were suggested to be conserved in dicistroviruses. The most N-terminal PSIV cleavage site was aa 286-287 (QS). Including previous results, the polyprotein contains nine proteins arranged as follows: 2A, 2B, 2C, 3A, 3B1, 3B2, 3B3, 3C, and 3D.
MeSH terms
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3C Viral Proteases
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Amino Acid Motifs
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Amino Acid Sequence
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Base Sequence
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Cysteine Endopeptidases / chemistry
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Cysteine Endopeptidases / genetics
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Cysteine Endopeptidases / metabolism*
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Dicistroviridae / chemistry
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Dicistroviridae / enzymology*
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Dicistroviridae / genetics
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Molecular Sequence Data
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Polyproteins / chemistry*
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Polyproteins / genetics
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Polyproteins / metabolism*
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Protein Processing, Post-Translational
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Sequence Alignment
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Viral Nonstructural Proteins / chemistry*
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Viral Nonstructural Proteins / genetics
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Viral Nonstructural Proteins / metabolism*
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Viral Proteins / chemistry
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Viral Proteins / genetics
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Viral Proteins / metabolism*
Substances
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Polyproteins
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Viral Nonstructural Proteins
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Viral Proteins
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Cysteine Endopeptidases
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3C Viral Proteases