Synthesis and in vitro biocompatibility assessment of a poly(amic acid) derived from ethylenediaminetetraacetic dianhydride

J Biomater Sci Polym Ed. 2011;22(4-6):683-700. doi: 10.1163/092050610X490149. Epub 2010 Jun 21.

Abstract

Poly(amic acid) (PAA) derived from ethylenediaminetetracetic dianhydride shows great potential as a biomaterial suitable for biomedical applications. To evaluate this polymer class further, in vitro cell toxicity (WST-1/ECS, ELISA based) and cell compatibility (cell adhesion and cell proliferation) tests were conducted to establish structure-toxicity relationships. PAAs with a number-average molecular weight ranging between 100 to 200 kg/mol were synthesized at 37°C after 24 h. Porcine radial artery cells (RACs) and descending aorta endothelial cells (ECs) were seeded independently in a 96-well cell culture plate at a cell density of 5000 cells/cm(2) to observe toxic effects. Similarly, RACs and ECs were seeded independently onto PAA coated and uncoated cover slips at a cell density of 7000 cells/cm(2) to observe growth patterns. Our results showed no toxicity after 96 h of incubation and in addition, both RACs and ECs adhered and proliferated on the PAA films, preserving their phenotype during this time. The tested synthetic material seems promising as a future biomaterial and should elicit a desired cellular response upon implantation.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anhydrides* / chemical synthesis
  • Anhydrides* / chemistry
  • Anhydrides* / metabolism
  • Animals
  • Benzene Derivatives* / chemical synthesis
  • Benzene Derivatives* / chemistry
  • Benzene Derivatives* / metabolism
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / metabolism
  • Calorimetry, Differential Scanning
  • Cell Adhesion
  • Cell Proliferation
  • Cell Survival
  • Cells, Cultured
  • Chelating Agents / chemical synthesis
  • Chelating Agents / chemistry
  • Chelating Agents / metabolism
  • Edetic Acid* / chemical synthesis
  • Edetic Acid* / chemistry
  • Edetic Acid* / metabolism
  • Endothelial Cells / cytology
  • Endothelial Cells / physiology
  • Materials Testing
  • Microscopy, Electron, Scanning / methods
  • Molecular Structure
  • Molecular Weight
  • Polymers* / chemical synthesis
  • Polymers* / chemistry
  • Polymers* / metabolism
  • Spectroscopy, Fourier Transform Infrared
  • Swine

Substances

  • Anhydrides
  • Benzene Derivatives
  • Biocompatible Materials
  • Chelating Agents
  • Polymers
  • polyamic acid
  • Edetic Acid