Abstract
This study was designed to investigate whether secretory-IgA (S-IgA) Abs induced by a pneumococcal surface protein A (PspA)-based nasal vaccine are necessary for prevention of streptococcal colonization. Mice nasally immunized with PspA plus a plasmid expressing Flt3 ligand (pFL) cDNA as a mucosal adjuvant showed significantly higher levels of PspA-specific S-IgA and IgG Ab responses in both plasma and nasal washes when compared with naive mice. Although IgA(-/-) mice given nasal PspA plus pFL had significantly high levels of PspA-specific IgG Abs, high numbers of CFUs were detected in nasal washes and nasal passages. In contrast, vaccinated wild-type mice showed essentially no bacteria in the nasal cavity. Further, a nasal vaccine consisting of PspA plus pFL effectively reduced pre-existing Streptococcus pneumoniae in the nasal cavity. These results show that PspA-based vaccine-induced specific S-IgA Abs play a necessary role in the regulation of S. pneumoniae colonization in the nasal cavity.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / administration & dosage
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Adjuvants, Immunologic / genetics
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Administration, Intranasal
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Animals
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Antibodies, Bacterial / biosynthesis
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Antibodies, Bacterial / physiology*
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Bacterial Proteins / administration & dosage
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Bacterial Proteins / genetics
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Bacterial Proteins / immunology*
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Bacterial Vaccines / administration & dosage
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Bacterial Vaccines / genetics
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Bacterial Vaccines / immunology
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Cells, Cultured
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Colony Count, Microbial
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Female
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Immunity, Innate* / genetics
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Immunoglobulin A, Secretory / biosynthesis
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Immunoglobulin A, Secretory / genetics
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Immunoglobulin A, Secretory / physiology*
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Membrane Proteins / administration & dosage
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Membrane Proteins / biosynthesis
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Membrane Proteins / genetics
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Membrane Proteins / immunology
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Streptococcal Infections / immunology
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Streptococcal Infections / microbiology
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Streptococcal Infections / prevention & control*
Substances
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Adjuvants, Immunologic
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Antibodies, Bacterial
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Bacterial Proteins
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Bacterial Vaccines
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Immunoglobulin A, Secretory
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Membrane Proteins
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flt3 ligand protein
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pneumococcal surface protein A