Advanced microscopy techniques for quantitative analysis in neuromorphology and neuropathology research: current status and requirements for the future

J Chem Neuroanat. 2010 Nov;40(3):199-209. doi: 10.1016/j.jchemneu.2010.06.005. Epub 2010 Jun 25.

Abstract

Visualizing neuromorphology and in particular neuropathology has been the focus of many researchers in the quest to solve the numerous questions that are still remaining related to several neurological and neuropsychiatric diseases. Over the last years, intense research into microscopy techniques has resulted in the development of various new types of microscopes, software imaging systems, and analysis programs. This review briefly discusses some key techniques, such as confocal stereology and automated neuron tracing and reconstruction, and their applications in neuroscience research. Special emphasis is placed on needs for further developments, such as the demand for higher-throughput analyses in quantitative neuromorphology. These developments will advance basic neuroscience research as well as pharmaceutical and biotechnology research and development.

Publication types

  • Review

MeSH terms

  • Animals
  • Forecasting
  • Humans
  • Image Processing, Computer-Assisted / methods
  • Microscopy / methods*
  • Microscopy / trends*
  • Neurology / methods*
  • Neurology / trends*
  • Neurons / ultrastructure*
  • Research