A Gram-staining-negative, non-motile, yellow-pigmented bacterial strain, designated HMD1043(T), was isolated from a mesotrophic artificial lake in Korea. The major fatty acids were anteiso-C(15 : 0) (28.3 %), iso-C(15 : 0) (22.9 %), summed feature 9 (comprising iso-C(17 : 1)ω9c and/or 10-methyl C(16 : 0); 8.8 %) and iso-C(13 : 0) (5.3 %). The DNA G+C content was 31.3 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMD1043(T) formed a lineage within the genus Chryseobacterium and was most closely related to Chryseobacterium antarcticum AT1013(T) (96.8 % 16S rRNA gene sequence similarity) and Chryseobacterium jeonii AT1047(T) (96.4 %). On the basis of the evidence presented in this study, strain HMD1043(T) is described as belonging to a novel species of the genus Chryseobacterium, for which the name Chryseobacterium yonginense sp. nov. is proposed. The type strain is HMD1043(T) ( = KCTC 22744(T) = CECT 7547(T)).