High-efficiency somatic reprogramming induced by intact MII oocytes

Cell Res. 2010 Sep;20(9):1034-42. doi: 10.1038/cr.2010.97. Epub 2010 Jul 6.

Abstract

Somatic nuclei can be reprogrammed into a pluripotent state by nuclear transfer, cell fusion and expression of transcription factors. However, these reprogramming processes are very inefficient, which has greatly hindered efforts to elucidate the underlying molecular mechanisms. Here, we report a new reprogramming strategy that combines the advantages of all three reprogramming methodologies into one process. We injected nuclei from cumulus cells into intact MII oocytes. Following activation, 80% of the reconstructed embryos developed to the blastocyst stage, and tetraploid (4N) embryonic stem (ES) cell lines were generated at a rate of 30% per reconstructed oocyte. We also generated triploid (3N) ES cells after injection of somatic nuclei into activated oocytes. 4N and 3N ES cells expressed pluripotent markers and differentiated into cell types of three embryonic germ layers in vivo. Moreover, all ES cells generated histocompatible, differentiated cells after being engrafted in immunocompetent B6D2F1 mice, showing that ES cells derived from this reprogramming strategy might serve as a source of genetically tailored tissues for transplantation. Thus, we have established a simple and highly efficient reprogramming procedure that provides a system for investigating the molecular mechanisms involved in somatic reprogramming.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastocyst / cytology
  • Cell Differentiation
  • Cellular Reprogramming*
  • Cumulus Cells
  • DNA Methylation
  • Embryonic Stem Cells / cytology
  • Immunocompromised Host
  • Metaphase
  • Mice
  • Mice, Transgenic
  • Nuclear Transfer Techniques
  • Oocytes / cytology*
  • Tetraploidy