[Development of a multiplex technique for detecting simultaneously HAV RNA and HEV RNA]

C Merviel; J-M Mansuy; M Dubois; J Izopet

doi:10.1016/j.patbio.2010.04.004

[Development of a multiplex technique for detecting simultaneously HAV RNA and HEV RNA]

Pathol Biol (Paris). 2012 Apr;60(2):95-105. doi: 10.1016/j.patbio.2010.04.004. Epub 2010 Jul 6.

[Article in French]

Authors

C Merviel¹, J-M Mansuy, M Dubois, J Izopet

Affiliation

¹ CHU de Toulouse, place du Docteur-Baylac, TSA 40031, 31059 Toulouse cedex 9, France. [email protected]

PMID: 20609530
DOI: 10.1016/j.patbio.2010.04.004

Abstract

The hepatitis E virus (HEV) is more and more frequently incriminated in hepatitis episodes. In non-endemic regions, it is clear now that this infection is autochthonous, and certainly a zoonosis and thus, that this virus must not only be assessed in hepatitis cases among travellers in endemic regions. In parallel, in endemic region, where HEV, like HAV, is mainly water-transmitted, important outbreaks still occur. This article describes the development and validation of a new molecular technique for detecting simultaneously HAV RNA and HEV RNA and its evaluation on clinical specimen.

Publication types

Evaluation Study
Validation Study

MeSH terms

Cross Reactions
False Positive Reactions
Hepatitis A / diagnosis*
Hepatitis A / genetics
Hepatitis A / virology
Hepatitis A virus / genetics*
Hepatitis A virus / isolation & purification
Hepatitis E / diagnosis*
Hepatitis E / genetics
Hepatitis E / virology
Hepatitis E virus / genetics*
Hepatitis E virus / isolation & purification
Humans
Limit of Detection
Multiplex Polymerase Chain Reaction / methods*
RNA, Viral / analysis
RNA, Viral / genetics
RNA, Viral / isolation & purification*
Reagent Kits, Diagnostic
Reproducibility of Results
Sensitivity and Specificity

Substances

RNA, Viral
Reagent Kits, Diagnostic