Fukutin mutations in congenital muscular dystrophies with defective glycosylation of dystroglycan in Korea

Neuromuscul Disord. 2010 Aug;20(8):524-30. doi: 10.1016/j.nmd.2010.06.005.

Abstract

This study was aimed to identify Fukutin (FKTN)-related congenital muscular dystrophies (CMD) with defective alpha-dystroglycan glycosylation in Korea and to discuss their genotype-phenotype spectrum focusing on detailed brain magnetic resonance imaging (MRI) findings. FKTN mutations were found in nine of the 12 CMD patients with defective alpha-dystroglycan glycosylation patients (75%). Two patients were homozygous for the Japanese founder retrotransposal insertion mutation. Seven patients were heterozygous for the retrotransposal insertion mutation, five of whom carried a novel intronic mutation that activates a pseudoexon between exons 5 and 6 (c.647+2084G>T). Compared with individuals that were homozygous for the retrotransposal insertion mutation, the seven heterozygotes for the retrotransposal insertion mutation, including five patients with the novel pseudoexon mutation, exhibited a more severe clinical phenotype in terms of motor abilities and more extensive brain MRI abnormalities (i.e., a wider distribution of cortical malformation and pons and cerebellar hypoplasia). FKTN mutations are the most common genetic cause of CMD with defective alpha-dystroglycan glycosylation in Korea. Compound heterozygosity of the retrotransposal insertion and the novel pseudoexon mutation is the most prevalent genotype in Korea and is associated with a more severe clinical and radiological phenotype compared with homozygosity for the retrotransposal insertion mutation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brain / pathology
  • Child
  • Child, Preschool
  • DNA / genetics
  • DNA Mutational Analysis
  • Dystroglycans / genetics*
  • Dystroglycans / metabolism
  • Exons / genetics
  • Female
  • Gene Frequency
  • Glycosylation
  • Heterozygote
  • Homozygote
  • Humans
  • Immunohistochemistry
  • Introns / genetics
  • Magnetic Resonance Imaging
  • Male
  • Membrane Proteins / genetics*
  • Muscle, Skeletal / metabolism
  • Muscle, Skeletal / pathology
  • Muscular Dystrophies / genetics*
  • Muscular Dystrophies / metabolism
  • Mutagenesis, Insertional
  • Phenotype
  • Republic of Korea / epidemiology
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • FKTN protein, human
  • Membrane Proteins
  • Dystroglycans
  • DNA