FISH mapping of six genes responsible for development of the nervous and skeletal systems on donkey (Equus asinus) chromosomes

Hereditas. 2010 Jun 1;147(3):132-5. doi: 10.1111/j.1601-5223.2010.02178.x.

Abstract

The results obtained in the present study made it possible to place selected markers responsible for development of the nervous and skeletal systems on the physical map of the donkey genome. Fluorescence in situ hybridization (FISH) was used to localize genes such as GDF5 (15q13), FRZB (4q23.1), TWIST (1q31), PAX6 (20q25), SALL1 (24q15) and SHH (1q35) on donkey chromosomes. The identification of their localization confirmed previously proposed homologies using ZOO-FISH technique, except for FRZB and SALL1 genes. This suggests that they were affected by rearrangements that changed their localization compared to horse, and in the case of the SALL1 gene also compared to human.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Chromosome Mapping
  • Chromosomes / genetics*
  • Equidae / genetics*
  • Eye Proteins / genetics
  • Genes / genetics*
  • Glycoproteins / genetics
  • Growth Differentiation Factor 5 / genetics
  • Hedgehog Proteins / genetics
  • Homeodomain Proteins / genetics
  • Humans
  • In Situ Hybridization, Fluorescence*
  • Intracellular Signaling Peptides and Proteins
  • Musculoskeletal Development / genetics*
  • Nervous System / growth & development*
  • PAX6 Transcription Factor
  • Paired Box Transcription Factors / genetics
  • Repressor Proteins / genetics
  • Transcription Factors / genetics
  • Twist-Related Protein 1 / genetics

Substances

  • Eye Proteins
  • Glycoproteins
  • Growth Differentiation Factor 5
  • Hedgehog Proteins
  • Homeodomain Proteins
  • Intracellular Signaling Peptides and Proteins
  • PAX6 Transcription Factor
  • PAX6 protein, human
  • Paired Box Transcription Factors
  • Repressor Proteins
  • Transcription Factors
  • Twist-Related Protein 1
  • WD repeat containing planar cell polarity effector