The structural plasticity of SCA7 domains defines their differential nucleosome-binding properties

EMBO Rep. 2010 Aug;11(8):612-8. doi: 10.1038/embor.2010.98. Epub 2010 Jul 16.

Abstract

SAGA (Spt-Ada-Gcn5 acetyltransferase), a coactivator complex involved in chromatin remodelling, harbours both histone acetylation and deubiquitination activities. ATXN7/Sgf73 and ATXN7L3, two subunits of the SAGA deubiquitination module, contain an SCA7 domain characterized by an atypical zinc-finger. We show that the yeast Sgf73-SCA7 domain is not required to recruit Sgf73 into SAGA. Instead, it binds to nucleosomes, a property that is conserved in the human ATXN7-SCA7 domain but is lost in the ATXN7L3 domain. The solution structures of the SCA7 domain of both ATXN7 and ATXN7L3 reveal a new, common zinc-finger motif at the heart of two distinct folds, providing a molecular basis for the observed functional differences.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Ataxin-7
  • Humans
  • Models, Molecular
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry*
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Nuclear Magnetic Resonance, Biomolecular
  • Nucleosomes / metabolism*
  • Protein Binding
  • Protein Structure, Secondary*
  • Protein Structure, Tertiary
  • Sequence Alignment
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Ubiquitination
  • Zinc Fingers

Substances

  • ATXN7 protein, human
  • ATXN7L3 protein, human
  • Ataxin-7
  • Nerve Tissue Proteins
  • Nucleosomes
  • Transcription Factors