Abstract
Heat-shock proteins (Hsps) are molecular chaperones that control protein folding and function. Argonaute 2 (Ago2), the effector in RNA interference (RNAi), is associated with Hsp90; however, its function in RNAi remains elusive. Here we show that Hsp90 is required for Ago2 to receive the small interfering RNA (siRNA) duplex from the RNA-induced silencing complex-loading complex in RNAi, suggesting a model where Hsp90 modifies Ago2 conformation to accommodate the siRNA duplex.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Drosophila Proteins / metabolism*
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Drosophila melanogaster / metabolism*
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HSP90 Heat-Shock Proteins / metabolism*
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Heat-Shock Proteins / metabolism*
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Molecular Sequence Data
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RNA Interference
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RNA Processing, Post-Transcriptional
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RNA, Small Interfering / genetics
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RNA, Small Interfering / metabolism
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RNA-Induced Silencing Complex / metabolism*
Substances
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Drosophila Proteins
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HSP90 Heat-Shock Proteins
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Heat-Shock Proteins
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Hsp83 protein, Drosophila
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RNA, Small Interfering
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RNA-Induced Silencing Complex