Design of 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde as a reagent for ultrasensitive determination of primary amines by capillary electrophoresis using laser fluorescence detection

Anal Chem. 1991 Mar 1;63(5):408-12. doi: 10.1021/ac00005a004.

Abstract

Amino acids and peptides, from both standard solutions and biological samples, were successfully reacted with 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde, at low concentration, to form highly fluorescent isoindole derivatives. The formed mixtures are effectively separated by high-performance capillary electrophoresis and their constituents detected by their laser-induced fluorescence signals. The minimum detectable quantities in the low attomole (10(-18) mol) range are encountered.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amines / analysis*
  • Amino Acids / analysis
  • Benzoates / chemistry*
  • Electrophoresis
  • Hydrogen-Ion Concentration
  • Indicators and Reagents
  • Lasers
  • Peptides / analysis
  • Quinolines / chemistry*
  • Spectrometry, Fluorescence

Substances

  • Amines
  • Amino Acids
  • Benzoates
  • Indicators and Reagents
  • Peptides
  • Quinolines
  • 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde