Abstract
We had shown that virus resistance to ADS-J1 was associated with amino acid changes in the envelope glycoprotein, mostly located in the gp120 coding region. Time-of-addition and endocytic virus transfer assays clearly demonstrated that ADS-J1 behaved as a gp120 inhibitor. ADS-J1-resistant virus was cross-resistant to the polyanion dextran sulfate, and recombination of gp120 recovered only the ADS-J1-resistant phenotype. In summary, ADS-J1 blocks an early step of virus entry that appears to be driven by gp120 alone.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-HIV Agents / metabolism
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Anti-HIV Agents / pharmacology*
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Cell Line
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HIV Envelope Protein gp120 / metabolism*
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HIV Envelope Protein gp41 / metabolism*
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HIV-1 / drug effects*
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HIV-1 / metabolism*
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Humans
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Naphthalenesulfonates / metabolism
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Naphthalenesulfonates / pharmacology*
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Triazines / metabolism
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Triazines / pharmacology*
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Virus Internalization / drug effects*
Substances
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Anti-HIV Agents
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HIV Envelope Protein gp120
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HIV Envelope Protein gp41
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Naphthalenesulfonates
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Triazines
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ADS J1