Analysis of candidate genes on chromosome 20q12-13.1 reveals evidence for BMI mediated association of PREX1 with type 2 diabetes in European Americans

Genomics. 2010 Oct;96(4):211-9. doi: 10.1016/j.ygeno.2010.07.006. Epub 2010 Jul 30.

Abstract

Chromosome 20q12-q13.1 has been linked to type 2 diabetes (T2D) in multiple populations. We examined the influence of genes in this region on T2D and BMI in two European American case-control populations. SNPs were genotyped in 300 diabetic patients and 310 controls. A subset of 72 SNPs were further genotyped in 470 cases and 442 controls. All genes examined showed evidence of association with T2D in the initial sample (additive P-value [P(a)]=0.00090-0.045). SNPs near PREX1 were also associated in the second case-control population (P(a)=0.017-0.042). The combined analysis resulted in the same SNPs, among others, associated with T2D (P(a)=0.0013-0.041). Stratification analysis by T2D status showed that association with BMI was observed solely in cases (P(a)=0.0018-0.041). Mediation testing revealed that 30-40% of the effects of these SNPs on T2D were significantly mediated by BMI. SNPs near PREX1 may contribute to T2D susceptibility mediated through effects of adiposity in European Americans.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adiposity / genetics
  • Adiposity / physiology
  • Americas
  • Body Mass Index*
  • Case-Control Studies
  • Chromosomes, Human, Pair 20* / genetics
  • Diabetes Mellitus, Type 2 / complications
  • Diabetes Mellitus, Type 2 / ethnology
  • Diabetes Mellitus, Type 2 / genetics*
  • Gene Frequency
  • Genetic Association Studies
  • Genetic Predisposition to Disease
  • Guanine Nucleotide Exchange Factors / genetics*
  • Humans
  • Kidney Failure, Chronic / complications
  • Kidney Failure, Chronic / genetics
  • Linkage Disequilibrium
  • Middle Aged
  • Polymorphism, Single Nucleotide
  • White People / genetics*

Substances

  • Guanine Nucleotide Exchange Factors
  • PREX1 protein, human