Preliminary data on effects of metformin on PED/PEA-15 cellular levels in obese women with polycystic ovary syndrome

J Endocrinol Invest. 2010 Jul-Aug;33(7):446-50. doi: 10.1007/BF03346622.

Abstract

Background: The cellular abundance of the phosphoprotein enriched in diabetes (PED/PEA-15), a 15 kDa protein related to insulin resistance (IR), is increased in women with polycystic ovary syndrome (PCOS).

Aim: To investigate whether metformin (MET) has additive effects on PED/PEA-15 protein levels.

Material/subjects and methods: This is an open label, prospective clinical study over 6 months. Ten hyperandrogenic obese PCOS women [age: 24.6+/-1.6 yr; body mass index (BMI): 30.7+/-1.2 kg/m(2)] were treated with MET (1250 mg/day). Ten age- and BMI-matched normo-androgenic women were used as controls. Outcome measures are: PED/PEA-15 protein levels, fasting plasma glucose and insulin (FPI), reciprocal index of homeostasis model assessment of insulin resistance (1/HOMA-IR); quantitative insulin sensitivity check index (QUICKI); wholebody insulin sensitivity index (ISI); SHBG; total testosterone; free androgen index (FAI).

Results: At baseline FPI and PED/PEA- 15 protein levels were higher, while 1/HOMA-IR, QUICKI, and ISI were lower (p<0.001) in MET group than in controls. After treatment, independently of body weight and hyperandrogenism, FPI, and PED/PEA-15 protein levels decreased (p=0.001 and 0.004, respectively), while, 1/HOMA-IR, QUICKI, and ISI increased (p<0.001). PED/PEA-15 protein levels correlated significantly with ISI either before (r=0.636; p=0.048), and after treatment (r=0.758; p=0.011).

Conclusions: PED/PEA-15 protein levels reduced after a short course of treatment with MET in a group hyperandrogenic obese PCOS women. This effect was independent of body weight and hyperandrogenism, and correlated with ISI, thus adding a further benefit to obese PCOS women.

Trial registration: ClinicalTrials.gov NCT00948402.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Androgens / blood
  • Apoptosis Regulatory Proteins
  • Blood Glucose / metabolism
  • Female
  • Humans
  • Insulin / blood
  • Insulin Resistance / physiology*
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Metformin / therapeutic use*
  • Obesity / blood
  • Phosphoproteins / metabolism*
  • Polycystic Ovary Syndrome / drug therapy*
  • Polycystic Ovary Syndrome / physiopathology
  • Sex Hormone-Binding Globulin / metabolism

Substances

  • Androgens
  • Apoptosis Regulatory Proteins
  • Blood Glucose
  • Insulin
  • Intracellular Signaling Peptides and Proteins
  • PEA15 protein, human
  • Phosphoproteins
  • Sex Hormone-Binding Globulin
  • Metformin

Associated data

  • ClinicalTrials.gov/NCT00948402