Remodeling genomes with artificial transcription factors (ATFs)

Methods Mol Biol. 2010:649:163-82. doi: 10.1007/978-1-60761-753-2_10.

Abstract

Chromatin structure plays a pivotal role in defining which regions of the genome are accessible for effective transcription. Chromatin-remodeling agents are able to relax this structure, facilitating the access of transcription factors into the DNA. Herein, we describe a new method, which combines artificial transcription factors (ATFs) and chromatin-remodeling agents to specifically reactivate silenced regions of the genome and reprogram cellular phenotypes.

MeSH terms

  • Apoptosis / drug effects
  • Apoptosis / genetics
  • Azacitidine / pharmacokinetics
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Chromatin / genetics
  • Chromatin / metabolism
  • Epigenesis, Genetic / genetics
  • Flow Cytometry
  • Humans
  • Models, Biological
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Zinc Fingers / genetics

Substances

  • Chromatin
  • Transcription Factors
  • Azacitidine