Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum beta-lactamases

Hiran Dhanji; Michel Doumith; Olivier Clermont; Erick Denamur; Russell Hope; David M Livermore; Neil Woodford

doi:10.1016/j.ijantimicag.2010.06.007

Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum beta-lactamases

Int J Antimicrob Agents. 2010 Oct;36(4):355-8. doi: 10.1016/j.ijantimicag.2010.06.007. Epub 2010 Aug 5.

Authors

Hiran Dhanji¹, Michel Doumith, Olivier Clermont, Erick Denamur, Russell Hope, David M Livermore, Neil Woodford

Affiliation

¹ Antibiotic Resistance Monitoring and Reference Laboratory, Centre for Infections, Health Protection Agency, London NW9 5EQ, UK. [email protected]

PMID: 20691573
DOI: 10.1016/j.ijantimicag.2010.06.007

Abstract

CTX-M-15 has become the most prevalent extended-spectrum beta-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify bla(CTX-M-15)-like.

Publication types

Evaluation Study

MeSH terms

Bacterial Typing Techniques
DNA, Bacterial / genetics
Epidemics*
Escherichia coli / classification*
Escherichia coli / enzymology
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Escherichia coli Infections / epidemiology*
Escherichia coli Infections / microbiology
Escherichia coli Proteins / biosynthesis
Escherichia coli Proteins / genetics
Humans
Molecular Epidemiology
Polymerase Chain Reaction / methods*
Polymorphism, Single Nucleotide
beta-Lactamases / biosynthesis
beta-Lactamases / genetics*

Substances

DNA, Bacterial
Escherichia coli Proteins
beta-lactamase CTX-M-15
beta-Lactamases