Abstract
AKAP79/150 is a protein scaffold that is thought to position specific kinases (protein kinase A and C) and phosphatases (calcineurin) in appropriate synaptic domains so that their activities can regulate excitatory synaptic strength. Using a viral-mediated molecular replacement strategy in rat hippocampal slices, we found that AKAP is required for NMDA receptor-dependent long-term depression solely because of its interaction with calcineurin.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
A Kinase Anchor Proteins / genetics
-
A Kinase Anchor Proteins / metabolism*
-
Animals
-
CA1 Region, Hippocampal / physiology
-
Calcineurin / metabolism*
-
Cells, Cultured
-
Excitatory Postsynaptic Potentials / physiology
-
In Vitro Techniques
-
Long-Term Synaptic Depression / physiology*
-
Neurons / physiology*
-
Pyramidal Cells / physiology
-
Rats
-
Rats, Sprague-Dawley
-
Receptors, AMPA / metabolism
-
Receptors, Metabotropic Glutamate / metabolism
-
Receptors, N-Methyl-D-Aspartate / metabolism*
-
Synaptic Transmission / physiology
Substances
-
A Kinase Anchor Proteins
-
Akap5 protein, rat
-
Receptors, AMPA
-
Receptors, Metabotropic Glutamate
-
Receptors, N-Methyl-D-Aspartate
-
Calcineurin