Abstract
We have developed a highly effective marker set for assigning medaka mutants to specific linkage groups (LG) in bulk segregation analysis by PCR product length polymorphisms (PLPs). The marker set permits analysis using an automated microchip electrophoresis system as well as conventional agarose gel electrophoresis.
MeSH terms
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Animals
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Automation
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Chromosome Mapping / methods*
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Chromosome Segregation / genetics
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Electrophoresis, Agar Gel / methods*
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Electrophoresis, Microchip / methods*
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Genetic Markers
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Mutation / genetics*
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Oryzias / genetics*
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Polymerase Chain Reaction / methods*
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Polymorphism, Restriction Fragment Length / genetics*