Abstract
Erythrocyte binding antigen region II (EBA-175) is a conserved antigen of Plasmodium falciparum that is involved in binding of the parasite to the host's erythrocytes. We evaluated the safety and immunogenicity of a recombinant EBA-175 vaccine with aluminum phosphate adjuvant in healthy young adults living in the United States. Eighteen subjects/group received ascending doses (5, 20, 80, or 160 μg) of the vaccine at 0, 1, and 6 months; 8 subjects received placebo. Most of the injection site and systemic reactions were mild to moderate in intensity. After 2 or 3 doses of the vaccine at any concentration, antibody levels measured by enzyme-linked immunosorbent assay were significantly higher than those for the placebo group. Sera from subjects who received 3 doses of the vaccine at any concentration inhibited the growth of erythrocyte-stage P. falciparum at low levels compared to sera from placebo recipients or preimmune sera. In conclusion, the EBA-175 vaccine with adjuvant was safe and immunogenic in malaria-naïve subjects.
Publication types
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Randomized Controlled Trial
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Research Support, N.I.H., Extramural
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Research Support, N.I.H., Intramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / administration & dosage
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Adolescent
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Adult
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Aluminum Compounds / administration & dosage
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Antibodies, Protozoan / blood
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Antigens, Protozoan / administration & dosage
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Antigens, Protozoan / adverse effects*
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Antigens, Protozoan / immunology*
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Enzyme-Linked Immunosorbent Assay
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Female
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Human Experimentation
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Humans
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Immunization, Secondary / methods
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Malaria Vaccines / administration & dosage
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Malaria Vaccines / adverse effects*
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Malaria Vaccines / immunology*
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Malaria, Falciparum / prevention & control*
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Male
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Phosphates / administration & dosage
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Placebos / administration & dosage
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Plasmodium falciparum / growth & development
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Plasmodium falciparum / immunology
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Protozoan Proteins / administration & dosage
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Protozoan Proteins / adverse effects*
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Protozoan Proteins / immunology*
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United States
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Vaccination / methods
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Vaccines, Synthetic / administration & dosage
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Vaccines, Synthetic / adverse effects
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Vaccines, Synthetic / immunology
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Young Adult
Substances
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Adjuvants, Immunologic
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Aluminum Compounds
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Antibodies, Protozoan
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Antigens, Protozoan
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Malaria Vaccines
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Phosphates
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Placebos
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Protozoan Proteins
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Vaccines, Synthetic
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erythrocyte-binding antigen 175, Plasmodium
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aluminum phosphate