A new method for labelling of monoclonal antibodies and their fragments with technetium-99m

E Joiris; B Bastin; J R Thornback

doi:10.1016/0883-2897(91)90131-4

A new method for labelling of monoclonal antibodies and their fragments with technetium-99m

Int J Rad Appl Instrum B. 1991;18(3):353-6. doi: 10.1016/0883-2897(91)90131-4.

Authors

E Joiris¹, B Bastin, J R Thornback

Affiliation

¹ R/D Imaging, Medgenix Group, Fleurus, Belgium.

PMID: 2071447
DOI: 10.1016/0883-2897(91)90131-4

Abstract

A new method of labelling of antibodies with technetium-99m is described. The antibody or fragment is derivatized by iminothiolane. Ligand exchange from a labile technetium complex such as TcO(GLU)2- results in greater than 90% of the technetium labelling the antibody. This method appears to be equally applicable to antibodies and fragments.

MeSH terms

Antibodies, Monoclonal / chemistry*
Chromatography, High Pressure Liquid
Fibrin / immunology
Humans
Imidoesters / chemistry
Immunoglobulin Fragments / chemistry
Immunoglobulin G / chemistry
Isotope Labeling
Technetium / chemistry*

Substances

Antibodies, Monoclonal
Imidoesters
Immunoglobulin Fragments
Immunoglobulin G
methyl 4-mercaptobutyrimidate
Technetium
Fibrin