First attempt to validate the gSG6-P1 salivary peptide as an immuno-epidemiological tool for evaluating human exposure to Anopheles funestus bites

Trop Med Int Health. 2010 Oct;15(10):1198-203. doi: 10.1111/j.1365-3156.2010.02611.x. Epub 2010 Aug 17.

Abstract

Summary objective: The development of a biomarker of exposure based on the evaluation of the human antibody response specific to Anopheles salivary proteins seems promising in improving malaria control. The IgG response specific to the gSG6-P1 peptide has already been validated as a biomarker of An. gambiae exposure. This study represents a first attempt to validate the gSG6-P1 peptide as an epidemiological tool evaluating exposure to An. funestus bites, the second main malaria vector in sub-Saharan Africa.

Methods: A multi-disciplinary survey was performed in a Senegalese village where An. funestus represents the principal anopheline species. The IgG antibody level specific to gSG6-P1 was evaluated and compared in the same children before, at the peak and after the rainy season.

Results: Two-thirds of the children developed a specific IgG response to gSG6-P1 during the study period and--more interestingly--before the rainy season, when An. funestus was the only anopheline species reported. The specific IgG response increased during the An. funestus exposure season, and a positive association between the IgG level and the level of exposure to An. funestus bites was observed.

Conclusions: The results suggest that the evaluation of the IgG response specific to gSG6-P1 in children could also represent a biomarker of exposure to An. funestus bites. The availability of such a biomarker evaluating the exposure to both main Plasmodium falciparum vectors in Africa could be particularly relevant as a direct criterion for the evaluation of the efficacy of vector control strategies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anopheles / immunology*
  • Biomarkers / blood
  • Child
  • Child, Preschool
  • Cohort Studies
  • Female
  • Humans
  • Immunoglobulin G / blood*
  • Infant
  • Insect Bites and Stings / diagnosis
  • Insect Bites and Stings / immunology*
  • Insect Proteins / immunology*
  • Longitudinal Studies
  • Male
  • Salivary Proteins and Peptides / immunology*
  • Senegal

Substances

  • Biomarkers
  • Immunoglobulin G
  • Insect Proteins
  • Salivary Proteins and Peptides
  • gSG6-P1 peptide, human