Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides

J Chromatogr A. 2010 Sep 17;1217(38):5978-85. doi: 10.1016/j.chroma.2010.07.067. Epub 2010 Aug 3.

Abstract

Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0 m(2)/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylamides / chemistry*
  • Chromatography, Liquid / methods*
  • Hydrophobic and Hydrophilic Interactions
  • Insulin / isolation & purification
  • Microspheres*
  • Molecular Weight
  • Nitrogen
  • Peptide Fragments / isolation & purification*
  • Polymethacrylic Acids / chemistry*
  • Porosity
  • Surface Properties
  • Temperature

Substances

  • Acrylamides
  • Insulin
  • Peptide Fragments
  • Polymethacrylic Acids
  • poly(N-isopropylacrylamide-N-tert-butylacrylamide)copolymer
  • Nitrogen