A novel DNA vaccine constructed by heat shock protein 70 and melanoma antigen-encoding gene 3 against tumorigenesis

Ping Qu; Jia-Hai Ma; Xiu-Min Zhang; Xiao-Jun Huang; Xin-Wei Yang; Sui Yan-Fang

A novel DNA vaccine constructed by heat shock protein 70 and melanoma antigen-encoding gene 3 against tumorigenesis

Indian J Exp Biol. 2010 May;48(5):436-43.

Authors

Ping Qu¹, Jia-Hai Ma, Xiu-Min Zhang, Xiao-Jun Huang, Xin-Wei Yang, Sui Yan-Fang

Affiliation

¹ Center of Teaching Experiment, School of Basic Medical Sciences, Fourth Military Medical University, Xi'an, Shaanxi Province 710032, PR China.

PMID: 20795360

Abstract

Melanoma antigen-encoding gene 3 (MAGE-3) is an ideal candidate for a tumor vaccine although its potency need to be increased. Heat shock proteins (HSPs) represents a potential approach for increasing the potency of DNA vaccines. In the present study, a fusion DNA vaccine composed of Mycobacterium tuberculosis HSP70 and MAGE-3 was constructed and used to immunize C57BL/6 mice against B16 or B16-MAGE-3 tumor cells. The results show that the HSP70-MAGE-3 fusion DNA vaccine enhanced the frequency of MAGE-3-specific cytotoxic T-cells as compared to the MAGE-3 DNA vaccine or the HSP70/MAGE-3 cocktail DNA vaccine (P < 0.05). In conclusion, the results indicate that the HSP70-MAGE-3 fusion DNA vaccine can strongly activate MAGE-3 specific cellular immunological reactions and thus significantly inhibit the growth of B16-MAGE-3 tumors, improving the survival of tumor-bearing mice, and the HSP70-MAGE-3 fusion DNA vaccine has a significant therapeutic effect on the tumors that express MAGE-3 antigens.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Neoplasm / biosynthesis
Antigens, Neoplasm / genetics
Antigens, Neoplasm / immunology*
Bacterial Proteins / genetics
Bacterial Proteins / immunology*
Cancer Vaccines / administration & dosage
Cancer Vaccines / immunology*
Cancer Vaccines / therapeutic use
Cytotoxicity, Immunologic
Drug Screening Assays, Antitumor
HSP70 Heat-Shock Proteins / genetics
HSP70 Heat-Shock Proteins / immunology*
Humans
Immunotherapy, Active
Injections, Intramuscular
Interferon-gamma / metabolism
Melanoma, Experimental / therapy
Mice
Mice, Inbred C57BL
Neoplasm Proteins / genetics
Neoplasm Proteins / immunology*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / immunology
T-Lymphocytes, Cytotoxic / immunology*
Vaccines, DNA / administration & dosage
Vaccines, DNA / immunology*
Vaccines, DNA / therapeutic use

Substances

Antibodies, Neoplasm
Antigens, Neoplasm
Bacterial Proteins
Cancer Vaccines
HSP70 Heat-Shock Proteins
HSP70 protein, Mycobacterium tuberculosis
MAGEA3 protein, human
Neoplasm Proteins
Recombinant Fusion Proteins
Vaccines, DNA
Interferon-gamma