Mutational spectrum of cystic fibrosis in the Lebanese population

J Cyst Fibros. 2010 Dec;9(6):406-10. doi: 10.1016/j.jcf.2010.08.001. Epub 2010 Aug 25.

Abstract

Background: Cystic fibrosis (CF) is the most common autosomal recessive disease in Caucasians; it is however, considered to be rare in the Arab populations. Reports of the cystic fibrosis transmembrane regulator (CFTR) mutations from Arabs, especially from the Lebanese population, are limited.

Methods: Twenty-two unrelated Lebanese families, with at least one child with CF, were studied. DNA extracts from blood samples of patients and parents were screened for CFTR gene mutations.

Results: Eleven different mutations were identified. Of the 44 alleles studied, the most common mutations were: F508del (34%), N1303K (27%), W1282X (7%), and S4X (7%). Five mutations - not previously reported in the Lebanese population - were identified; these are: S549N, G542X, 2043delG, 4016insG, and R117H-7T.

Conclusions: The most common CFTR mutations in addition to five mutations not previously described in the Lebanese population were identified. Identification of CFTR mutations in the Lebanese population is important for molecular investigations and genetic counseling.

MeSH terms

  • Arabs / genetics*
  • Arabs / statistics & numerical data*
  • Child
  • Child, Preschool
  • Cystic Fibrosis / ethnology*
  • Cystic Fibrosis / genetics*
  • Cystic Fibrosis Transmembrane Conductance Regulator / genetics*
  • DNA Mutational Analysis
  • Female
  • Genetic Counseling
  • Humans
  • Infant
  • Infant, Newborn
  • Lebanon / epidemiology
  • Male
  • Young Adult

Substances

  • CFTR protein, human
  • Cystic Fibrosis Transmembrane Conductance Regulator