Abstract
Age-related metabolic changes in the hippocampus of APP(Swe)/PS1(dE9) mice were measured with long echo-time in vivo (1)H-magnetic resonance spectroscopy ((1)H-MRS). Thioflavine S staining and Nissl staining were used to characterize deposition of Aβ aggregates and neuronal degeneration in the transgenic animals, respectively. The results showed that the APP(Swe)/PS1(dE9) mice had significantly decreased hippocampal N-acetyl aspartate (NAA)/total creatine (tCr) level at 16 months of age, which was associated with degeneration of and intracellular deposition of thioflavine S-positive materials in hippocampal CA3 pyramidal neurons. The results of this study provide direct evidence showing association among Aβ pathology (intracellular deposition of thioflavine S-positive materials), neuronal degeneration, and metabolic changes observable with in vivo (1)H-MRS in the hippocampus of APP(Swe)/PS1(dE9) mice.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aging / metabolism*
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Aging / pathology
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Alzheimer Disease / genetics
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Alzheimer Disease / metabolism
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Alzheimer Disease / pathology
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Amyloid beta-Protein Precursor / genetics*
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Amyloid beta-Protein Precursor / metabolism
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Animals
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Aspartic Acid / analogs & derivatives*
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Aspartic Acid / metabolism
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Benzothiazoles
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CA1 Region, Hippocampal / metabolism*
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CA1 Region, Hippocampal / pathology
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Creatine / metabolism
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Disease Models, Animal
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Down-Regulation / genetics*
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Female
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Humans
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Magnetic Resonance Spectroscopy / methods
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Male
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Mice
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Mice, Transgenic
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Nerve Degeneration / metabolism*
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Nerve Degeneration / pathology
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Plaque, Amyloid / metabolism
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Plaque, Amyloid / pathology
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Presenilin-1 / genetics*
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Presenilin-1 / metabolism
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Pyramidal Cells / metabolism*
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Pyramidal Cells / pathology
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Pyramidal Cells / physiopathology
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Thiazoles
Substances
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Amyloid beta-Protein Precursor
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Benzothiazoles
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PSEN1 protein, human
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Presenilin-1
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Thiazoles
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thioflavin T
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Aspartic Acid
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N-acetylaspartate
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Creatine