Enhancement of the diversity of polyoxins by a thymine-7-hydroxylase homolog outside the polyoxin biosynthesis gene cluster

Changming Zhao; Tingting Huang; Wenqing Chen; Zixin Deng

doi:10.1128/AEM.01257-10

Enhancement of the diversity of polyoxins by a thymine-7-hydroxylase homolog outside the polyoxin biosynthesis gene cluster

Appl Environ Microbiol. 2010 Nov;76(21):7343-7. doi: 10.1128/AEM.01257-10. Epub 2010 Sep 3.

Authors

Changming Zhao¹, Tingting Huang, Wenqing Chen, Zixin Deng

Affiliation

¹ Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200030, China.

Abstract

Polyoxins consist of 14 structurally variable components which differentiate at three branch sites of the carbon skeleton. Open reading frame (ORF) SAV_4805 of Streptomyces avermitilis, showing similarity to thymine-7-hydroxylase, was proved to enhance the diversity of polyoxins at the C-5 site of the 1-(5'-amino-5'-deoxy-β-d-allofuranuronosyl) pyrimidine moiety.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antifungal Agents / metabolism
Metabolic Networks and Pathways / genetics
Mixed Function Oxygenases / genetics*
Mixed Function Oxygenases / metabolism
Multigene Family / genetics
Open Reading Frames / genetics
Pyrimidine Nucleosides / biosynthesis
Pyrimidine Nucleosides / chemistry
Pyrimidine Nucleosides / genetics
Sequence Homology, Amino Acid
Streptomyces / enzymology
Streptomyces / genetics
Streptomyces / metabolism

Substances

Antifungal Agents
Pyrimidine Nucleosides
polyoxin
Mixed Function Oxygenases
thymine, 2-oxoglutarate dioxygenase