Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strain

S B Santos; E Fernandes; C M Carvalho; S Sillankorva; V N Krylov; E A Pleteneva; O V Shaburova; A Nicolau; E C Ferreira; J Azeredo

doi:10.1128/AEM.00922-10

Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strain

Appl Environ Microbiol. 2010 Nov;76(21):7338-42. doi: 10.1128/AEM.00922-10. Epub 2010 Sep 3.

Authors

S B Santos¹, E Fernandes, C M Carvalho, S Sillankorva, V N Krylov, E A Pleteneva, O V Shaburova, A Nicolau, E C Ferreira, J Azeredo

Affiliation

¹ Institute for Biotechnology and Bioengineering, Center of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4700-057 Braga, Portugal.

Abstract

We report the selection and amplification of the broad-host-range Salmonella phage phi PVP-SE1 in an alternative nonpathogenic host. The lytic spectrum and the phage DNA restriction profile were not modified upon replication in Escherichia coli Bl21, suggesting the possibility of producing this phage in a nonpathogenic host, contributing to the safety and easier approval of a product based on this Salmonella biocontrol agent.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Escherichia coli / virology*
Humans
Microscopy, Electron, Transmission
Phenotype
Polymorphism, Restriction Fragment Length / genetics
Salmonella Infections / prevention & control
Salmonella Infections, Animal / prevention & control
Salmonella Phages / genetics
Salmonella Phages / growth & development
Salmonella Phages / pathogenicity*
Salmonella Phages / physiology
Salmonella Phages / ultrastructure
Salmonella enterica / virology