Development of SCAR markers and PCR assays for single or simultaneous species-specific detection of Phytophthora nicotianae and Pythium helicoides in ebb-and-flow irrigated kalanchoe

Monday O Ahonsi; Yin Ling; Koji Kageyama

doi:10.1016/j.mimet.2010.08.019

Development of SCAR markers and PCR assays for single or simultaneous species-specific detection of Phytophthora nicotianae and Pythium helicoides in ebb-and-flow irrigated kalanchoe

J Microbiol Methods. 2010 Nov;83(2):260-5. doi: 10.1016/j.mimet.2010.08.019. Epub 2010 Sep 15.

Authors

Monday O Ahonsi¹, Yin Ling, Koji Kageyama

Affiliation

¹ River Basin Research Center, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. [email protected]

PMID: 20826191
DOI: 10.1016/j.mimet.2010.08.019

Abstract

Phytophthora nicotianae and Pythium helicoides are important water-borne oomycete pathogens of irrigated ornamentals particularly ebb-and-flow irrigated kalanchoe in Japan. We developed novel PCR-based sequence characterized amplified region markers and assays for rapid identification and species-specific detection of both pathogens in separate PCR reactions or simultaneously in a duplex PCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Fungal / chemistry
DNA, Fungal / genetics
Japan
Kalanchoe / microbiology*
Molecular Sequence Data
Mycology / methods*
Phytophthora / genetics
Phytophthora / isolation & purification*
Plant Diseases / microbiology*
Polymerase Chain Reaction / methods*
Pythium / genetics
Pythium / isolation & purification*
Sequence Analysis, DNA

Substances

DNA, Fungal

Associated data

GENBANK/GQ857069
GENBANK/GQ857070
GENBANK/GQ857071
GENBANK/GQ857072
GENBANK/GQ857073