DNA-nanoparticle micelles as supramolecular fluorogenic substrates enabling catalytic signal amplification and detection by DNAzyme probes

Miao-Ping Chien; Matthew P Thompson; Nathan C Gianneschi

doi:10.1039/c0cc02291h

DNA-nanoparticle micelles as supramolecular fluorogenic substrates enabling catalytic signal amplification and detection by DNAzyme probes

Chem Commun (Camb). 2011 Jan 7;47(1):167-9. doi: 10.1039/c0cc02291h. Epub 2010 Sep 7.

Authors

Miao-Ping Chien¹, Matthew P Thompson, Nathan C Gianneschi

Affiliation

¹ Department of Chemistry & Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.

PMID: 20830351
DOI: 10.1039/c0cc02291h

Abstract

Catalytic DNA molecules have tremendous potential in propagating detection events via nucleic acid sequence selective signal amplification. However, they suffer from product inhibition limiting their widespread utility. Herein, this limitation is overcome utilizing a novel fluorogenic substrate design consisting of cooperatively assembled DNA-nanoparticle micelles.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Binding Sites
Biocatalysis
DNA, Catalytic / chemistry*
DNA, Catalytic / metabolism*
DNA, Single-Stranded / chemistry*
Fluorescent Dyes / chemistry*
Macromolecular Substances / chemistry
Micelles*
Molecular Structure
Nanoparticles / chemistry*
Nucleic Acid Amplification Techniques*
Particle Size
Substrate Specificity

Substances

DNA, Catalytic
DNA, Single-Stranded
Fluorescent Dyes
Macromolecular Substances
Micelles