Lipopolysaccharide from Burkholderia thailandensis E264 provides protection in a murine model of melioidosis

Vaccine. 2010 Nov 3;28(47):7551-5. doi: 10.1016/j.vaccine.2010.08.058. Epub 2010 Sep 15.

Abstract

Burkholderia thailandensis is a less virulent close relative of Burkholderia pseudomallei, a CDC category B biothreat agent. We have previously shown that lipopolysaccharide (LPS) extracted from B. pseudomallei can provide protection against a lethal challenge of B. pseudomallei in a mouse model of melioidosis. Sugar analysis on LPS from B. thailandensis strain E264 confirmed that this polysaccharide has a similar structure to LPS from B. pseudomallei. Mice were immunised with LPS from B. thailandensis or B. pseudomallei and challenged with a lethal dose of B. pseudomallei strain K96243. Similar protection levels were observed when either LPS was used as the immunogen. This data suggests that B. thailandensis LPS has the potential to be used as part of a subunit based vaccine against pathogenic B. pseudomallei.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Bacterial / blood
  • Antigens, Bacterial / immunology
  • Antigens, Bacterial / isolation & purification
  • Bacterial Vaccines / immunology
  • Burkholderia / immunology
  • Burkholderia / pathogenicity*
  • Burkholderia pseudomallei / immunology
  • Burkholderia pseudomallei / pathogenicity*
  • Disease Models, Animal
  • Female
  • Lipopolysaccharides / immunology*
  • Lipopolysaccharides / isolation & purification
  • Melioidosis / immunology
  • Melioidosis / prevention & control*
  • Mice
  • Mice, Inbred BALB C

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Vaccines
  • Lipopolysaccharides