Genetic transformation and mutagenesis via single-stranded DNA in the unicellular, diazotrophic cyanobacteria of the genus Cyanothece

Appl Environ Microbiol. 2010 Nov;76(22):7641-5. doi: 10.1128/AEM.01456-10. Epub 2010 Sep 17.

Abstract

We describe a genetic system for producing specific gene knockouts in Cyanothece sp. strain PCC 7822 using a single-stranded DNA technique (B. Zorin, P. Hegemann, and I. Sizova, Eukaryot. Cell 4:1264-1272, 2005). The first fully segregated mutant was a ΔnifK mutant, and it was unable to grow on medium lacking combined nitrogen and produced virtually no hydrogen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Culture Media / chemistry
  • Cyanothece / genetics*
  • DNA, Single-Stranded / genetics*
  • Gene Knockout Techniques / methods*
  • Genes, Bacterial
  • Genetics, Microbial / methods*
  • Hydrogen / metabolism
  • Mutagenesis*
  • Transformation, Genetic*

Substances

  • Culture Media
  • DNA, Single-Stranded
  • Hydrogen