Monocyte activation by apoptotic cells removal in systemic lupus erythematosus patients

Cell Immunol. 2010;266(1):52-60. doi: 10.1016/j.cellimm.2010.08.012. Epub 2010 Sep 21.

Abstract

Decreased apoptotic cells (ACs) removal has been described as relevant in systemic lupus erythematosus (SLE) pathogenesis. Binding/phagocytosis of ACs was decreased in SLE patients. Blocking experiments suggested a role for CD36 in ACs clearance in healthy controls, not observed in SLE patients. Binding/phagocytosis of ACs induced the production of IL-6, CXCL8 and CCL22 in patients and controls and IL-1β, TNF-α and CCL3 only in healthy controls. ACs clearance induced an increase in CD80 and a decrease in CD86 expression in healthy controls and atherosclerotic patients. However, SLE patients did not up-regulate CD80 expression. The number and expression of CD36 and CD163 in monocytes was not different between the groups. ACs removal induced a down-regulation of CD36 expression in adherent HLA-DR(+) cells in SLE patients but not healthy controls. The decreased binding/phagocytosis of ACs observed in SLE patients, induces a distinct immune response compared with healthy controls.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / pharmacology
  • Antigens, CD / immunology
  • Antigens, CD / metabolism
  • Antigens, Differentiation, Myelomonocytic / immunology
  • Antigens, Differentiation, Myelomonocytic / metabolism
  • Apoptosis / immunology*
  • Atherosclerosis / immunology
  • B7-1 Antigen / metabolism
  • B7-2 Antigen / metabolism
  • CD36 Antigens / immunology
  • CD36 Antigens / metabolism
  • CD40 Antigens / metabolism
  • Chemokine CCL2 / metabolism
  • Chemokine CCL22 / metabolism
  • Chemokine CCL3 / metabolism
  • Culture Media, Conditioned / metabolism
  • Female
  • HLA-DR Antigens / metabolism
  • Humans
  • Interleukin-1beta / metabolism
  • Interleukin-6 / metabolism
  • Interleukin-8 / metabolism
  • Leukocytes, Mononuclear / immunology
  • Leukocytes, Mononuclear / metabolism
  • Lupus Erythematosus, Systemic / immunology*
  • Male
  • Middle Aged
  • Monocytes / cytology
  • Monocytes / immunology*
  • Monocytes / metabolism
  • Phagocytosis / drug effects
  • Phagocytosis / immunology*
  • Receptors, Cell Surface / immunology
  • Receptors, Cell Surface / metabolism
  • Tumor Necrosis Factor-alpha / metabolism
  • Young Adult

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • B7-1 Antigen
  • B7-2 Antigen
  • CCL2 protein, human
  • CCL22 protein, human
  • CCL3 protein, human
  • CD163 antigen
  • CD36 Antigens
  • CD40 Antigens
  • CD86 protein, human
  • CXCL8 protein, human
  • Chemokine CCL2
  • Chemokine CCL22
  • Chemokine CCL3
  • Culture Media, Conditioned
  • HLA-DR Antigens
  • IL6 protein, human
  • Interleukin-1beta
  • Interleukin-6
  • Interleukin-8
  • Receptors, Cell Surface
  • Tumor Necrosis Factor-alpha