Emergence of oseltamivir-resistant pandemic (H1N1) 2009 virus within 48 hours

Masafumi Inoue; Timothy Barkham; Yee Sin Leo; Kwai Peng Chan; Angela Chow; Christopher W Wong; Raphael Tze Chuen Lee; Sebastian Maurer-Stroh; Raymond Lin; Cui Lin

doi:10.3201/eid1610.100688

Emergence of oseltamivir-resistant pandemic (H1N1) 2009 virus within 48 hours

Emerg Infect Dis. 2010 Oct;16(10):1633-6. doi: 10.3201/eid1610.100688.

Authors

Masafumi Inoue¹, Timothy Barkham, Yee Sin Leo, Kwai Peng Chan, Angela Chow, Christopher W Wong, Raphael Tze Chuen Lee, Sebastian Maurer-Stroh, Raymond Lin, Cui Lin

Affiliation

¹ Agency for Science, Research and Technology, Singapore.

Abstract

An oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus evolved and emerged from zero to 52% of detectable virus within 48 hours of a patient's exposure to oseltamivir. Phylogenetic analysis and data gathered by pyrosequencing and cloning directly on clinical samples suggest that the mutant emerged de novo.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Antiviral Agents / pharmacology*
Drug Resistance, Viral / genetics*
Evolution, Molecular
Female
Humans
Influenza A Virus, H1N1 Subtype / drug effects*
Influenza A Virus, H1N1 Subtype / genetics
Influenza A Virus, H1N1 Subtype / isolation & purification
Influenza A Virus, H1N1 Subtype / pathogenicity
Influenza, Human / drug therapy*
Influenza, Human / epidemiology
Influenza, Human / virology
Mutation*
Oseltamivir / pharmacology*
Pandemics
Phylogeny
Sequence Analysis, DNA / methods
Time Factors

Substances

Antiviral Agents
Oseltamivir