The complete mitochondrial genome of Radix balthica (Pulmonata, Basommatophora), obtained by low coverage shot gun next generation sequencing

B Feldmeyer; K Hoffmeier; M Pfenninger

doi:10.1016/j.ympev.2010.09.012

The complete mitochondrial genome of Radix balthica (Pulmonata, Basommatophora), obtained by low coverage shot gun next generation sequencing

Mol Phylogenet Evol. 2010 Dec;57(3):1329-33. doi: 10.1016/j.ympev.2010.09.012. Epub 2010 Sep 27.

Authors

B Feldmeyer¹, K Hoffmeier, M Pfenninger

Affiliation

¹ Biodiversity and Climate Research Center (BiK-F), Siesmayerstr. 70A, 60323 Frankfurt am Main, Germany. [email protected]

PMID: 20875865
DOI: 10.1016/j.ympev.2010.09.012

Abstract

A 454-FLX low-coverage sequencing approach was used to assemble the mitochondrial genome of Radix balthica. The mtDNA sequence is 13,993 nt long and contains 37 genes (13 protein coding genes, two rRNAs and 22 tRNAs). Four genes, the 12S RNA and seven tRNAs are transcribed in reverse order. The sequence is AT rich (71.3%), similar to other basommatophoran species. Comparison with the most closely related mt genomes available (Biomphalaria glabrata and Biomphalaria tenagophila) revealed identical gene orders except for five tRNAs. Next generation sequencing proved to be a fast and easy method for sequencing an entire mitochondrial genome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Comparative Genomic Hybridization
DNA, Mitochondrial / genetics
Gene Order
Genome, Mitochondrial*
RNA, Transfer / genetics
Sequence Analysis, DNA / methods*
Snails / classification
Snails / genetics*

Substances

DNA, Mitochondrial
RNA, Transfer