Isolation and identification of islet amyloid polypeptide in normal human pancreas

M Nakazato; J Asai; M Miyazato; S Matsukura; K Kangawa; H Matsuo

doi:10.1016/0167-0115(90)90004-g

Isolation and identification of islet amyloid polypeptide in normal human pancreas

Regul Pept. 1990 Dec 10;31(3):179-86. doi: 10.1016/0167-0115(90)90004-g.

Authors

M Nakazato¹, J Asai, M Miyazato, S Matsukura, K Kangawa, H Matsuo

Affiliation

¹ Third Department of Internal Medicine, Miyazaki Medical College, Japan.

PMID: 2091067
DOI: 10.1016/0167-0115(90)90004-g

Abstract

To identify islet amyloid polypeptide (IAPP) present in normal human pancreas, we isolated the peptide from a soluble peptide fraction of amyloid deposit-free pancreata of two non-diabetic patients by using reverse-phase high performance liquid chromatography coupled with a radioimmunoassay specific for human IAPP. IAPP(1-37) and IAPP(17-37) were isolated and their complete amino acid sequences were determined up to the C-terminus. Identification of IAPP in normal human pancreas suggests the possible biological function of IAPP as a novel pancreatic hormone in humans.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Amino Acid Sequence
Amyloid / analysis
Amyloid / chemistry
Amyloid / isolation & purification*
Chromatography, High Pressure Liquid
Humans
Islet Amyloid Polypeptide
Islets of Langerhans / chemistry*
Male
Middle Aged
Molecular Sequence Data
Radioimmunoassay

Substances

Amyloid
Islet Amyloid Polypeptide