NF-kappaB inhibits T-cell activation-induced, p73-dependent cell death by induction of MDM2

Proc Natl Acad Sci U S A. 2010 Oct 19;107(42):18061-6. doi: 10.1073/pnas.1006163107. Epub 2010 Oct 4.

Abstract

NF-κB is a key transcription factor involved in the regulation of T-cell activation and proliferation upon engagement of the T-cell receptor (TCR). T cells that lack the IκB kinase (IKKβ) are unable to activate NF-κB, and rapidly undergo apoptosis upon activation. NF-κB activation following T-cell receptor engagement induces the expression of Mdm2 through interaction with NF-κB sites in its P1 promoter, and enforced expression of Mdm2 protected T cells deficient for NF-κB activation from activation-induced cell death. In T cells with intact NF-κB signaling, ablation or pharmacologic inhibition of Mdm2 resulted in activation-induced apoptosis. Mdm2 coprecipitates with p73 in activated T cells, and apoptosis induced by inhibition of Mdm2 was p73-dependent. Further, Bim was identified as a p73 target gene required for cell death induced by Mdm2 inhibition, and a p73-responsive element in intron 1 of Bim was characterized. Our results demonstrate a pathway for survival of activated T cells through NF-κB-induced Mdm2, which blocks Bim-dependent apoptosis through binding and inhibition of p73.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / physiology*
  • Apoptosis Regulatory Proteins / genetics
  • Bcl-2-Like Protein 11
  • DNA-Binding Proteins / physiology*
  • Lymphocyte Activation / physiology*
  • Membrane Proteins / genetics
  • Mice
  • Mice, Knockout
  • NF-kappa B / physiology*
  • Nuclear Proteins / physiology*
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins c-mdm2 / biosynthesis*
  • Proto-Oncogene Proteins c-mdm2 / genetics
  • T-Lymphocytes / cytology
  • T-Lymphocytes / metabolism*
  • Tumor Protein p73
  • Tumor Suppressor Proteins / physiology*

Substances

  • Apoptosis Regulatory Proteins
  • Bcl-2-Like Protein 11
  • Bcl2l11 protein, mouse
  • DNA-Binding Proteins
  • Membrane Proteins
  • NF-kappa B
  • Nuclear Proteins
  • Proto-Oncogene Proteins
  • Trp73 protein, mouse
  • Tumor Protein p73
  • Tumor Suppressor Proteins
  • Mdm2 protein, mouse
  • Proto-Oncogene Proteins c-mdm2