Abstract
While establishing a quantification standard for parvovirus B19 diagnostics, we extracted viral DNA from a high-titered human plasma by using three commercial kits. Despite similar viral DNA yields being obtained, striking differences in electrophoretic mobilities of the extracted nucleic acids were observed.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA, Viral / genetics*
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DNA, Viral / isolation & purification*
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Electrophoresis
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Humans
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Parvovirus B19, Human / genetics*
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Plasma / virology
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Reagent Kits, Diagnostic
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Specimen Handling / methods*
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Virology / methods*
Substances
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DNA, Viral
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Reagent Kits, Diagnostic